Paplašināta spektra β-laktamāzes kodējošo gēnu noteikšana Escherichia coli kultūrās, kas izolētas no mājas cūkām

6533b7dafe1ef96bd126dbe2

RESEARCH PRODUCT

Paplašināta spektra β-laktamāzes kodējošo gēnu noteikšana Escherichia coli kultūrās, kas izolētas no mājas cūkām

subject

Sus scrofa domestica antibiotic resistance Escherichia coli Bioloģija extended spectrum β-lactamases

description

Darba mērķis bija izpētīt Pārtikas drošības, dzīvnieku veselības un vides zinātniskajā institūtā „BIOR” izolētas Escherichia coli kultūras, nosakot TEM, OXA, SHV, CTX-M I, CTX-M II, CTX-M IV, CMY II un DHA tipa β-laktamāzes gēnu klātbūtni baktēriju kultūru paraugos. Bakalaura darbs izstrādāts 2017. gadā zinātniskā institūta “BIOR” Dzīvnieku slimību diagnostikas laboratorijas Mikrobioloģijas nodaļā un Molekulārās bioloģijas nodaļā. Pētāmie paraugi (Escherichia coli kultūras) bija iegūti no mājas cūku (Sus scrofa domestica) aklo zarnu paraugiem 2016. un 2017. gada VPP “AgroBioRes” ietvaros. Kopā tika analizēti 68 Escherichia coli kultūru paraugi no dažādām saimniecībām. β-lactamāzeskodējošo gēnu klātbūtne izolēto baktēriju genomā tika noteikta ar multipleksa PĶR metodi, pēc tam amplikoni tika sekvenēti, lai validētu iegūtos rezultātus. Atsevišķi tika noteikts izolēto baktēriju fenotips un antibiotiku sinerģija ar MIC testa palīdzību.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/37067

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