6533b7dafe1ef96bd126e303

RESEARCH PRODUCT

No evidence of DUI in the Mediterranean alien species Brachidontes pharaonis (P. Fisher, 1870) despite mitochondrial heteroplasmy

Marek LubośnyBeata ŚMietankaMarco ArculeoArtur Burzyński

subject

MaleMultidisciplinaryDUI mitochondrial haplogroups mitogenomes Brachidontes pharaonisSettore BIO/05 - ZoologiaHeteroplasmyDNA MitochondrialBivalviaGenome MitochondrialMustelidaeAnimalsHumansMytilidaeFemaleIntroduced Species

description

AbstractTwo genetically different mitochondrial haplogroups of Brachidontes pharaonis (p-distance 6.8%) have been identified in the Mediterranean Sea. This hinted at a possible presence of doubly uniparental inheritance in this species. To ascertain this possibility, we sequenced two complete mitogenomes of Brachidontes pharaonis mussels and performed a qPCR analysis to measure the relative mitogenome copy numbers of both mtDNAs. Despite the presence of two very similar regions composed entirely of repetitive sequences in the two haplogroups, no recombination between mitogenomes was detected. In heteroplasmic individuals, both mitogenomes were present in the generative tissues of both sexes, which argues against the presence of doubly uniparental inheritance in this species.

10.1038/s41598-022-12606-6https://hdl.handle.net/10447/589502