6533b7dafe1ef96bd126eb87
RESEARCH PRODUCT
A method for eluting DNA in a wide range of molecular weights from agarose gels
Giovanni GiudiceRainer BarbieriMaria CantoneGiovanni DuroMaria Assunta CostaVincenzo Izzosubject
Electrophoresis Agar GelGel electrophoresisChromatographyParafilmMolecular massElutionChemistryBiophysicsNucleic Acid HybridizationDNACell BiologyBiochemistryBuffer (optical fiber)Molecular Weightchemistry.chemical_compoundYield (chemistry)ElectrodeAgaroseMolecular Biologydescription
We have developed a simple and rapid method for recovering DNAs of a wide range of molecular weights from agarose gels. A DNA-containing gel slice is placed on a Parafilm sheet in the center of a circular (positive) electrode and covered with a drop of buffer, while a linear (negative) electrode is placed on the top of the gel and driven about 1 mm into the gel itself. When a continuous current is applied, the DNA migrates into the buffer toward the circular electrode. We have obtained almost total recovery of DNAs up to 10 kb in size. Our method may also be used, under appropriate conditions, for higher molecular weight DNAs. The yield and all the biological assays performed on the DNAs obtained by our method recommend it for routine laboratory use.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-05-15 | Analytical Biochemistry |