6533b7dafe1ef96bd126f577
RESEARCH PRODUCT
Conjugative plasmid pIP501 undergoes specific deletions after transfer from Lactococcus lactis to Oenococcus oeni
Isabel PardoManuel ZúñigaManuel ZúñigaSergi Ferrersubject
DNA BacterialMolecular Sequence DataRestriction Mappingmedicine.disease_causeBiochemistryMicrobiologyPlasmidGene OrderGeneticsmedicineAmino Acid SequenceMolecular BiologySequence DeletionOenococcus oeniGeneticsMutationBase SequencebiologyStrain (chemistry)Lactococcus lactisConjugative plasmidGeneral Medicinebiology.organism_classificationStreptococcaceaeGram-Positive CocciLactococcus lactisGenes BacterialConjugation GeneticGene DeletionLeuconostocBacteriaPlasmidsdescription
Conjugal transfer of plasmids pIP501 and its derivative pVA797 from Lactococcus lactis to Oenococcus oeni was assayed by filter mating. Plasmid pIP501 was transferred to a number of O. oeni strains whereas a single transconjugant of O. oeni M42 was recovered when pVA797 was used. Physical analysis of the transconjugant plasmids revealed that pIP501 and pVA797 underwent extensive deletions in O. oeni that affected the tra region (conjugal transfer) and SegB region (stability). All derivatives showed segregational instability in O. oeni, but were stably maintained in L. lactis. These differences correlated with the different plasmid copy numbers and the extent of deletions within the SegB region.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-11-01 | Archives of Microbiology |