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RESEARCH PRODUCT

Additional file 3: Figure S2. of The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae

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Workflow of populations’ selection and sequencing. Cells were grown in complete media (YPD) and synthetic must (SM), and were incubated at either optimum temperature (28 °C) or low temperature (15 °C) until the stationary phase was reached. At this time, the volume required to inoculate at an OD of 0.2 was re-inoculated into 60 mL of fresh medium. The experiment was carried out 8 times after which the selected populations were analyzed and sequenced. (PDF 43 kb)