6533b7dbfe1ef96bd1270166

RESEARCH PRODUCT

NFAT transcription factors control HIV-1 expression through a binding site downstream of TAR region.

Nadezhda RomanchikovaEriks JankevicsEdgar SerflingCarsten SchellerChristian JassoyVictorija Ivanova

subject

Transcription GeneticvirusesImmunologyTransfectionJurkat cellsJurkat CellsTranscription (biology)Immunology and AllergyHumansNuclear proteinBinding siteTranscription factorHIV Long Terminal RepeatBinding SitesNFATC Transcription FactorsChemistryNuclear ProteinsNFATHematologyU937 CellsNFATC Transcription FactorsMolecular biologyDNA-Binding Proteinscardiovascular systemHIV-1HIV Long Terminal RepeatTranscription Factors

description

NFAT factors control HIV-1 transcription. We show here that, in addition to binding to two NF-kappaB/NFAT sites within the U3 HIV LTR, NFATc1 and NFATc2 bind to an NFAT site within the LTR's U5 region. Mutations in this site which abolish NFAT binding reduce the ability of NFATs to transactivate LTR-mediated transcription. Mutations in all three NFAT sites strongly interfered with LTR induction, but affected moderately the stimulatory effect of Tat.

yearjournalcountryeditionlanguage
2004-01-30Immunobiology
10.1078/0171-2985-00283https://pubmed.ncbi.nlm.nih.gov/14748509