Transfekcijas efektivitāte HeLa, SKOV-3 un HPAF-II šūnu populācijās pēc to apstrādes ar UV starojumu

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Transfekcijas efektivitāte HeLa, SKOV-3 un HPAF-II šūnu populācijās pēc to apstrādes ar UV starojumu

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Darba mērķis bija noteikt transfekcijas efektivitāti HeLa, SKOV-3 un HPAF-II šūnu populācijās pēc to apstrādes ar UV starojumu. Pētījumā izmantotās šūnu kultūras tika apstarotas ar UV gaismu 90 min., pēc apstarošanas bija 24 un 48 stundu ilgs kontakts ar plazmīdu pGFP-N3, un līdz ar to tās tika kultivētas 24 un 48 stundas. Izmantojot fluorescences mikroskopiju, tika noteikta transfekcijas efektivitāte. Savstarpēji tika analizēts GFP ekspresējošo šūnu daudzums un ar DAPI krāsoto šūnu daudzums, kā arī procentuālais GFP ekspresējošo mikrošūnu daudzums un ar DAPI krāsoto mikrošūnu daudzums. Tika noteikts, ka visefektīvāk transfekcija noris, ja šūnas kultivē 24 stundas. Darbs izstrādāts Latvijas Biomedicīnas pētījumu un studiju centrā no 2012.gada septembra līdz 2013. gada aprīlim. Atslēgvārdi: HeLa, SKOV-3, HPAF-II, GFP, transfekcija, UV starojums.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/22944

PEPhysical Sciences and Engineering
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