6533b7dcfe1ef96bd1271f69
RESEARCH PRODUCT
Proeryptotic Activity of 4-Hydroxynonenal: A New Potential Physiopathological Role for Lipid Peroxidation Products
Ignazio RestivoAlessandro AttanzioAlessandro PitruzzellaLuisa TesoriereSonya VastoMario AllegraAlberto FucarinoMaria A. Livreasubject
Adult0301 basic medicineCeramideErythrocyteslcsh:QR1-502PhosphatidylserinesBiochemistryArticleRBClcsh:Microbiology4-HydroxynonenalLipid peroxidationprostaglandins03 medical and health scienceschemistry.chemical_compound0302 clinical medicineeryptosisCell AdhesionHuman Umbilical Vein Endothelial CellsHumansMolecular BiologyCells CulturedCaspaseAldehydesbiologyGlutathionePhosphatidylserineMiddle AgedIntercellular Adhesion Molecule-1Haemolysislipid peroxidation productsGlutathione4-hydroxynonenalCell biology030104 developmental biologychemistryinflammation030220 oncology & carcinogenesisbiology.proteinCalciumLipid PeroxidationIntracellulardescription
Background: Eryptosis is a physiological, apoptosis-like death of injured erythrocytes crucial to prevent premature haemolysis and the pathological sequalae generated by cell-free haemoglobin. When dysregulated, the process is associated to several inflammatory-based pathologies. 4-Hydroxy-trans-2-nonenal (HNE) is an endogenous signalling molecule at physiological levels and, at higher concentrations, is involved in the pathogenesis of several inflammatory-based diseases. This work evaluated whether HNE could induce eryptosis in human erythrocytes. Methods: Measurements of phosphatidylserine, cell volume, intracellular oxidants, Ca++, glutathione, ICAM-1, and ceramide were assessed by flow cytometry. Scanning electron microscopy evaluated morphological alterations of erythrocytes. Western blotting assessed caspases. PGE2 was measured by ELISA. Adhesion of erythrocytes on endothelial cells was evaluated by gravity adherence assay. Results: HNE in the concentration range between 10&ndash
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-05-01 | Biomolecules |