6533b7dcfe1ef96bd127212f
RESEARCH PRODUCT
Control of in vitro somatic embryogenesis of the spindle tree ( Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium
Laurent BonneauA. Biahouasubject
SucroseSomatic embryogenesisEmbryoPlant ScienceGeneral MedicineBiologybiology.organism_classificationIn vitrochemistry.chemical_compoundchemistryBiochemistryOsmotic pressureEuonymus europaeusSugarAgronomy and Crop ScienceExplant culturedescription
In vitro somatic embryogenesis was achieved from zygotic embryo explants of a woody angiosperm species, the spindle tree, cultivated on various culture media differing in their sugar type and concentration, or in the applied osmotic potential. The highest frequency of somatic embryogenesis was obtained with a 350 mM sucrose, or a 89 mM glucose concentration in the culture medium. Experiments with culture media differing only in osmotic potential indicated that a minimal threshold osmotic potential is required to stimulate the emergence of somatic embryos. Elevated concentrations of glucose have an inhibitory effect, independent of their osmotic effect, while elevated concentrations of sucrose mainly act osmotically, stimulating the emergence of numerous somatic embryos.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-12-30 | Plant Cell Reports |