Increasing voltage gradient electrophoresis of DNA

6533b7dcfe1ef96bd12721fc

RESEARCH PRODUCT

Increasing voltage gradient electrophoresis of DNA

D. Oliva Giorgia Sisino Rainer Barbieri Daniele Bellavia Sabina Di Maio

subject

Electrophoresis Agar Gel Gel electrophoresis Chromatography Organic Chemistry Voltage gradient DNA General Medicine Fractionation VGGE electrophoresis DNA Mitochondrial Biochemistry Analytical Chemistry Matrix (chemical analysis)chemistry.chemical_compound Electrophoresis Settore BIO/18 - Genetica chemistry Agarose Restriction fragment length polymorphism DNA

description

We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.

year	journal	country	edition	language
2007-12-10

http://hdl.handle.net/10447/35977

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