6533b7dcfe1ef96bd12727cd
RESEARCH PRODUCT
Studies on the fat body of oncopeltus fasciatus invaded by pseudomonas aeruginosa1
August Dornsubject
Programmed cell deathbiologyEndoplasmic reticulumCellMatrix (biology)biology.organism_classificationRibosomeMicrobiologymedicine.anatomical_structureCytoplasmLipid dropletmedicineEcology Evolution Behavior and SystematicsBacteriadescription
A fatal disease in laboratory cultures of Oncopeltus fasciatus is caused by Pseudomonas aeruginosa , which invades the fat body and destroys it. The cellular breakdown was studied by electron microscopy. Infected fat body tissue shrinks, disintegrates, and dissolves. The nuclei, apparently, remain intact until immediately before cell death. Bacteria, which are found singularly in cytoplasm, form an expanding electron-lucent halo. Within this halo, free ribosomes and ribosomes of rough endoplasmic reticulum (ER) disappear. The changes are presumably caused by proteolytic activity of the parasite, which results in the destruction of the fat body and death of the host. Lipid droplets dissolve, apparently pass into the matrix of cytoplasm, and may be discharged. The fate of carbohydrates is unclear. When whole clusters of bacteria colonize a cell, they are embedded in a stroma. Bundles of fibers are formed in and around it. They outlive the cellular breakdown and enclose numerous bacteria. The significance of the fiber production is not clear. Blood cells do not attack invading bacteria.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1977-01-01 | Journal of Invertebrate Pathology |