6533b7dcfe1ef96bd1273259

RESEARCH PRODUCT

Age-related changes in antioxidant status and oxidative damage to lipids and dna in mitochondria of rat liver

Guillermo T. SáezPilar MuñizC. PeiroVictoria Valls

subject

chemistry.chemical_classificationmedicine.medical_specialtyAntioxidantbiologyChemistryDNA damagemedicine.medical_treatmentGlutathione peroxidaseBioengineeringGlutathioneMitochondrionmedicine.disease_causeApplied Microbiology and BiotechnologyBiochemistrySuperoxide dismutaseLipid peroxidationchemistry.chemical_compoundEndocrinologyBiochemistryInternal medicinemedicinebiology.proteinOxidative stress

description

To investigate the correlation between oxidative stress and mitochondrial damage with aging, antioxidant system, levels of oxidative DNA damage and as an index of the loss of plasma membrane integrity lipid peroxidation and membrane potential were studied. Results showed that the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase significantly decreased during aging, however glutathione peroxidase (GSH-PX) increased in the aged mitochondria and glutathione (GSH) did not change during aging. No statistical difference was observed in the lipid peroxidation of mitochondria between young and old animals. The level of oxidative DNA damage (measured as 8oxo-dG) tended to increase during aging.

yearjournalcountryeditionlanguage
2005-02-01Process Biochemistry
https://doi.org/10.1016/j.procbio.2004.02.025