6533b7dcfe1ef96bd127328a

RESEARCH PRODUCT

Cavernolide

Inmaculada PosadasMaría Carmen TerencioS. De RosaMiguel Payá

subject

chemistry.chemical_classificationPhospholipase AbiologyDegranulationGeneral MedicineGeneral Biochemistry Genetics and Molecular BiologyNitric oxide synthaseManoalidechemistry.chemical_compoundEnzymechemistryBiochemistryMyeloperoxidasebiology.proteinNeutrophil degranulationTumor necrosis factor alphaGeneral Pharmacology Toxicology and Pharmaceutics

description

Abstract The inhibitory effect of cavernolide, a novel C 21 terpene lactone isolated from the sponge Fasciospongia cavernosa , on PLA 2 and other enzyme activities involved in the inflammatory process was studied. Cavernolide inhibited human synovial sPLA 2 in a concentration-dependent manner with an IC 50 value of 8.8 μM. Besides, this compound decreased in the nanomolar range the myeloperoxidase degranulation process using different stimuli. Cavernolide also inhibited TNFα, NO and PGE 2 production in intact cell experiments. NO and PGE 2 reduction was the consequence of the inhibition on iNOS and COX-2 expression because it did not affect inducible nitric oxide synthase and cyclooxygenase-2 activities in intact cells.

yearjournalcountryeditionlanguage
2000-11-01Life Sciences
https://doi.org/10.1016/s0024-3205(00)00875-4