6533b7dcfe1ef96bd1273417
RESEARCH PRODUCT
Small unilamellar liposomes from mixed natural and polymeric phospholipids: stability and susceptibility to phospholipase A2.
Margaret CritchlowJohn B. LloydDiane E. DaviesDavid W. GraingerAnke ReichertHelmut Ringsdorfsubject
BiophysicsPhospholipidSynthetic membraneTritiumBiochemistryPhospholipases Achemistry.chemical_compoundEndocrinologyPhospholipase A2MonolayerCarbon RadioisotopesPhospholipidsPhospholipase ALiposomeChromatographybiologyVesicleBilayertechnology industry and agricultureInulinTemperatureHydrogen-Ion ConcentrationPhospholipases A2GlucosechemistryLiposomesbiology.proteinlipids (amino acids peptides and proteins)Dimyristoylphosphatidylcholinedescription
The concept of the uncorkable liposome composed of phase-separated mixtures of a polymerized phospholipid and an enzymically digestible phospholipid has been investigated, using small unilamellar vesicles composed of mixtures of (polymerized) dienoylphosphatidylcholine (DENPC) and dimyristoylphosphatidylcholine (DMPC). Mixed liposomes, even those containing only 10% DENPC, were much more stable than DMPC liposomes, as indicated by the release of entrapped [3H]inulin or [14C]glucose. DMPC liposomes released entrapped solute on exposure to phospholipase A2, whereas mixed vesicles were resistant. The results are compared with those of an earlier study on monolayers of similar compositions. It is concluded that the liposomes, like the monolayers, are phase-mixed, and that uncorkable liposomes cannot be constructed from the phospholipid mixture employed. It is proposed that, until further experimental evidence is produced, the enzymatically uncorkable liposome must be regarded as a theoretical construct.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-06-19 | Biochimica et biophysica acta |