6533b7ddfe1ef96bd1273eff
RESEARCH PRODUCT
Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification
Giuseppe BarracoIsabelle SylvestreGiovanni IapichinoFlorent Engelmannsubject
CryopreservationSucroseChromatographyCallus formationLimoniumSettore AGR/04 - Orticoltura E FloricolturaHorticultureBiologyLimonium serotinumVitrification solutionbiology.organism_classificationCryopreservationchemistry.chemical_compoundCryopreservation Droplet-vitrification Limonium serotinum Statice Sucrose pretreatment Vitrification solutionchemistryDroplet-vitrificationShootBotanyGlycerolStaticeSucrose pretreatmentVitrificationEthylene glycoldescription
Abstract In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-07-01 |