6533b7ddfe1ef96bd1274ad3

RESEARCH PRODUCT

Selection of single-chain antibodies against the VP8* subunit of rotavirus VP4 outer capsid protein and their expression in Lactobacillus casei.

Jesús Rodríguez-díazRosa VianaJavier BuesaVicente MonederoGaspar Pérez-martínez

subject

RotavirusLactobacillus caseiPhage displayvirusesMolecular Sequence Datachemical and pharmacologic phenomenamedicine.disease_causeAntibodies ViralApplied Microbiology and BiotechnologyVirusMicrobiologyCell Linefluids and secretionsPeptide LibraryRotavirusmedicineHumansAmino Acid SequencePeptide libraryEcologybiologyfood and beveragesrespiratory systembiology.organism_classificationPhysiology and BiotechnologyVirologyComplementarity Determining RegionsIn vitroCulture MediaLacticaseibacillus caseiCapsidCapsid ProteinsSingle-Chain AntibodiesFood ScienceBiotechnology

description

ABSTRACTSingle-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression inLactobacillus caseiof one of the scFv were constructed.L. caseiwas able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

yearjournalcountryeditionlanguage
2004-11-06Applied and environmental microbiology
10.1128/aem.70.11.6936-6939.2004https://pubmed.ncbi.nlm.nih.gov/15528568