Zymomonas mobilis elpošanas ķēdes mutantu skābekļa un temperatūras stresa tolerance

6533b7ddfe1ef96bd1274cb3

RESEARCH PRODUCT

Zymomonas mobilis elpošanas ķēdes mutantu skābekļa un temperatūras stresa tolerance

Laura Istominova

subject

Termālais stress Zymomonas mobilis Elpošanas ķēde Bioloģija Oksidatīvais stress

description

Zymomonas mobilis Zm6 celms un astoņi tā elpošanas ķēdes mutantu celmi tika audzēti četros dažādos apstākļos: 30oC aerobi (kratītāja kolbās), 30oC mikroaerobos (bez kratīšanas), 42oC aerobi un 42oC mikroaerobi, lai salīdzinātu oksidatīvā un termālā stresa ietekmi uz katru no šiem celmiem. Tika noteikta celmu augšana, etanola produkcija un skābekļa patēriņš. Citohroma c peroksidāzes pārekspresija uzlaboja augšanu 30oC mikroaerobos apstākļos. Tomēr citohroma c peroksidāzes un NADH dehidrogenāzes negatīvajam dubultmutantam augšana 42oC aerobos apstākļos ir samazināta, salīdzinot ar NADH dehidrogenāzes negatīvo mutantu. Citohroma c peroksidāzes pārekspresija izraisīja ievērojamu skābekļa patēriņa samazināšanos, kas norāda uz jaunu, vēl nezināmu regulatoro efektu. NADH dehidrogenāzes negatīvajām mutantam bija paaugstināta termotolerance, bet laktātdehidrogenāzes un NADH dehidrogenāzes negatīvajā dubultmutantā šis efekts neizpaudās. Bakalaura darbs izstrādāts Latvijas Universitātes Mikrobioloģijas un Biotehnoloģijas institūtā.

year	journal	country	edition	language
2018-01-01

https://dspace.lu.lv/dspace/handle/7/38632

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