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RESEARCH PRODUCT
Methods for the Identification of Tainting Terpenoids and other Compounds from Algae
Erkki KolehmainenReijo KauppinenMirja LahtiperäAnja VeijanenJaakko Paasivirtasubject
SolventEnvironmental EngineeringChromatographyChemistryProton NMRThermal desorptionInfrared spectroscopyGas chromatographyFourier transform infrared spectroscopyMass spectrometryTerpenoidWater Science and Technologydescription
Off-flavour compounds produced by algae in freshwater ecosystems were studied for their structure using integrated sensory and spectroscopic methods: mass spectrometry (MS), nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR). Both solvent extraction and thermal desorption were used to isolate and to introduce the compounds into gas chromatography/mass spectrometry (GC/MS) and gas chromatography/Fourier transform infrared (GC/FTIR) systems. Ten ng of a terpenoid compound gave a readable IR spectrum. For 1H NMR studies the compounds were collected directly into NMR solvent by preparative gas chromatography. About 5 µg of a monoterpenoid compound was needed for a reliable 1H NMR spectrum. The mass spectral data indicated that the series of odorous substances detected by GC were terpenoid hydrocarbons and a later eluting series of compounds were sesquiterpene alcohols. In addition, one compound with an intense off-odour (aromatic, nitrogen containing compound) and some tainting carboxylic acids (derived from a starch factory) were detected.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-01-01 | Water Science and Technology |