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RESEARCH PRODUCT
Candida albicans UBI3 and UBI4 promoter regions confer differential regulation of invertase production to Saccharomyces cerevisiae cells in response to stress
P. RoigD. Gozalbosubject
Microbiology (medical)Hot TemperatureGlycoside HydrolasesSaccharomyces cerevisiaeRibosome biogenesisSaccharomyces cerevisiaeMicrobiology:CIENCIAS DE LA VIDA [UNESCO]:CIENCIAS DE LA VIDA::Microbiología [UNESCO]Gene Expression Regulation FungalCandida albicansUNESCO::CIENCIAS DE LA VIDAPromoter Regions GeneticCandida albicansUNESCO::CIENCIAS DE LA VIDA::MicrobiologíaUbiquitinsGeneRegulation of gene expressionbeta-FructofuranosidasebiologyPromoterbiology.organism_classificationMolecular biologyCell biologyInvertaseCandida albicans ; Ubiquitin genes ; Invertase ; Saccharomyces cerevisiae ; Promoter gene fusion ; Heterologous expressionInvertaseUbiquitin genesHeterologous expressionHeterologous expressionPromoter gene fusiondescription
Candida albicans ubiquitin genes UBI3 and UBI4 encode a ubiquitin-hybrid protein involved in ribosome biogenesis and polyubiquitin, respectively. In this work we show that UBI3 and UBI4 promoter regions confer differentialexpr ession consistent with the function of their encoded gene products. Hybrid genes were constructed containing the SUC2 coding region under the controlof UBI3 or UBI4 promoters in the yeast vector pLC7. Invertase production in Saccharomyces cerevisiae transformants was differentially regulated: the UBI4 promoter was induced by stress conditions (thermalupshift and/or starvation) whereas the UBI3 promoter conferred constitutive invertase production in growing yeast cells. These results indicate that the UBI4 promoter is regulated by stress-response signaling pathways, whereas the UBI3 promoter is controlled according to the requirement for protein synthesis to support cell growth. Gozalbo Flor, Daniel, Daniel.Gozalbo@uv.es
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-01-01 | International Microbiology |