6533b7ddfe1ef96bd1275569

RESEARCH PRODUCT

Molecular and taxonomic characterization of a endophytic fungus isolated from Helleborus bocconei subsp. intermedius (Ranunculaceae)

Marcella PasqualettiFrancesco Maria RaimondoVivienne Spadaro

subject

biologySettore BIO/02 - Botanica SistematicaHelleborusRanunculaceaePlant ScienceEndophytic fungusbiology.organism_classificationEndophyteMolecular analysisChaetomium strumariumChaetomium strumarium Chaetomiaceae endophyte endemic plant molecular analysis folk veterinary medicine.BotanySettore BIO/15 - Biologia FarmaceuticaChaetomiaceae

description

A non-sporulating fungus was isolated from different organs of Helleborus bocconei subsp. intermedius (Ranunculaceae) endemic to southern Italy and Sicily, known for the traditional use of dried roots in the treatment of lung diseases of cattle and horses. Molecular characterization of endophytic fungus based on the internal transcribed spacer (ITS) region of the rRNA gene sequences was done. The DNA sequence of full length ITS region of the studied fungus was a 100% match to that of Chaetomium strumarium strain dH 21642 (GenBank accession number JX280851.1). The morphological characters of colony and mycelium of this microfungus are reported here.

https://doi.org/10.7320/flmedit24.071