A method to isolate cDNA-quality RNA from adult conifer needles and a psbA cDNA from Norway spruce

6533b7defe1ef96bd1275b80

RESEARCH PRODUCT

A method to isolate cDNA-quality RNA from adult conifer needles and a psbA cDNA from Norway spruce

A. Sutter M. Relle Aloysius Wild

subject

Genetics Physiology Sequence analysis fungi Nucleic acid sequence RNA Plant Science Biology Stop codon Open reading frame Complementary DNA RNA extraction Agronomy and Crop Science Gene

description

Summary In order to investigate the expression of the psbA gene in damaged and undamaged Norway spruce trees ( Picea abies ) a cDNA clone encoding the D1 protein was isolated via RT-PCR. Applying a method developed by Schneiderbauer et al. (1991) with some modifications, we were able to obtain the required RNA from mature needles and successfully reverse transcribe it into cDNA. Sequence analysis of the cDNA clone revealed an open reading frame (ORF) encoding a 353 amino acid polypeptide that is highly homologous to the D1 protein sequences deduced from higher plant psbA genes. A 4 bp insertion, directly following the stop codon ochre (TAA), was found by comparison with two Pinus species, thus creating two additional potential stop codons.

year	journal	country	edition	language
1996-01-01	Journal of Plant Physiology

https://doi.org/10.1016/s0176-1617(96)80200-8

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