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RESEARCH PRODUCT

Sulphation and Hydrolysis Improvements of Bioactivities, and Immuno-Modulatory Properties of Edible Amanita hemibapha Subspecies javanica (Corner and Bas) Mucilage Polysaccharide as a Potential in Personalized Functional Foods

Noppol LeksawasdiPhisit SeesuriyachanYuthana PhimolsiripolFrancisco J. BarbaSutee WangtueaiUtoomporn SurayotWarawut KrusongSangguan YouCharin Techapun

subject

Microbiology (medical)sulphationQH301-705.5Plant SciencePolysaccharideArticlechemistry.chemical_compoundSulfationAmanita hemibaphamushroomAmanita hemibapha subspecies javanica (Corner and Bas)Biology (General)Protein kinase ACytotoxicityReceptorEcology Evolution Behavior and Systematicsimmunomodulatorychemistry.chemical_classificationbiologybiology.organism_classification<i>Amanita hemibapha</i> subspecies <i>javanica</i> (Corner and Bas)mucilage polysaccharideDEAE-SepharosechemistryPerforinBiochemistryhydrolysisbiology.protein<i>Amanita hemibapha </i>subspecies <i>javanica </i>(Corner and Bas)

description

In this study, the mucilage polysaccharide (MP) from Amanita hemibapha subspecies javanica was prepared by hot water extraction and ethanol precipitation and then fractionated using anion-exchange chromatography equipped with a DEAE Sepharose fast flow column. The most immune-enhancing polysaccharide fraction 2 (MPF2) was subjected to a structural modification such as hydrolysis or over-sulphation. The sulphate and molecular weight (Mw) of over-sulphated (OS1-3) and hydrolysed (HS1-3) derivatives of MPF2 differed between 9.85% and 14.2% and 32.8 and 88.1 × 103 g/mol, respectively. Further, the immune-enhancing properties of MPF2 and its derivatives were tested on RAW264.7 and NK cells through various in vitro assays. Interestingly, a low molecular weight of HS1-3 significantly increased the nitric oxide (NO) production (p &lt

yearjournalcountryeditionlanguage
2021-10-10Journal of Fungi
10.3390/jof7100847https://www.mdpi.com/2309-608X/7/10/847