Atgremotāju klīniskā materiāla Listeria innocua izolātu ģenētiskais raksturojums

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RESEARCH PRODUCT

Atgremotāju klīniskā materiāla Listeria innocua izolātu ģenētiskais raksturojums

Silva Gradovska

subject

Listerioze Bioloģija Atgremotāji Pilna genoma analīze Listeria innocua

description

Silva Gradovska (2019): Atgremotāju klīniskā materiāla Listeria innocua izolātu ģenētiskais raksturojums. Listeria ģints baktērijas ir plaši vidē un pārtikas produktos sastopamas baktērijas, no kurām daļa spēj ierosināt listeriozi. Listeria innocua ir Gram-pozitīva vides baktērija, kas ir radniecīga patogēnajai Listeria monocytogenes. Darba mērķis bija raksturot L. innocua izolātus, kas iegūti no atgremotāju klīniskā materiāla un, izmantojot pilna genoma sekvenēšanu, veikt genoma analīzi. Bakalaura darba izstrādes laikā no 70 Listeria innocua atgremotāju klīniskajiem izolātiem, kas ievākti laika periodā no 2012. līdz 2018. gadam, un no kuriem izdalīta DNS, tika sekvenēti ar Illumina MiSeq. Iegūtajos genomos tika novērtēta galveno Listeria spp. virulences faktoru klātbūtne, salīdzinot ar patogēnās L. monocytogenes virulences faktoriem. Piecos izolātos tika identificētas pilna Listeria patogenitātes saliņas-3 gēnu klasteris. Galvenais PrfA regulējošais virulences gēnu klasteris netika konstatēts nevienā no L. innocua izolātiem.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/48274

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