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RESEARCH PRODUCT
Rapid microarray-based typing of forensic SNPs
Magdalena BogusAngel CarracedoBeatriz SobrinoKlaus BenderPeter M. SchneiderPeter M. SchneiderPeter M. Schneidersubject
MicroarrayComplementary DNAMultiplex polymerase chain reactionGlass slideSingle-nucleotide polymorphismCTAGGeneral MedicineComputational biologyTypingBiologyDNA microarrayMolecular biologydescription
The single base extension-tag array (SBE-Tag Array) method is carried out on glass slides and combines the specificity of minisequencing for SNP typing with the high throughput capacity of microarrays. Following multiplex PCR, a single tube SBE reaction is carried out, and the fluorescent labelled extension products are hybridized to the complementary DNA sequence tag (cTag) immobilized on a glass slide for locus-specific laser scan analysis. The aim is to prove and optimise the conventional microarray reaction on accuracy and efficiency for forensic applications. © 2005 Elsevier B.V. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-04-01 | International Congress Series |