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RESEARCH PRODUCT

In vitro cytokine profile revealed differences from dorsal and ventral skin susceptibility to pathogen-probiotic interaction in gilthead seabream

María ÁNgeles EstebanHéctor CorderoManuela MauroAlberto CuestaMatteo Cammarata

subject

Fish Proteins0301 basic medicinemedicine.medical_treatmentGilthead seabream (Sparus aurata)Shewanella putrefaciensAquatic ScienceShewanella putrefaciensProbioticMicrobiologylaw.inventionFish Diseases03 medical and health sciencesProbioticlawmedicineAnimalsEnvironmental ChemistryCytokinePathogenSkinintegumentary systembiologyPathogenPhotobacteriumProbiotics04 agricultural and veterinary sciencesGeneral MedicinePhotobacteriumbiology.organism_classificationAnimal FeedSea BreamDiet030104 developmental biologyCytokinePhotobacterium damselae040102 fisheriesCytokines0401 agriculture forestry and fisheriesInterleukin 18Disease SusceptibilityGram-Negative Bacterial InfectionsBacteria

description

Skin is the first barrier of defense on fish, which is crucial to protection against different stressors, including pathogens. Skin samples obtained from dorsal and ventral part of Sparus aurata specimens were incubated with Photobacterium damselae subsp. piscicida (a pathogen for this fish species), with Shewanella putrefaciens Pdp11 (a probiotic bacteria isolated from healthy gilthead seabream skin) or with both bacteria. The gene expression profile of nine cytokines (il1b, tnfa, il6, il7, il8, il15, il18, il10 and tgfb) was studied by qPCR in all the skin samples. The present findings revealed different patterns of cytokine profile in dorsal and ventral skin of gilthead seabream, which could be related to the influence and susceptibility to a possible infection.

yearjournalcountryeditionlanguage
2016-04-27Fish & Shellfish Immunology
https://doi.org/10.1016/j.fsi.2016.07.018