6533b7defe1ef96bd127678c
RESEARCH PRODUCT
Dexamethasone dipropionate loaded nanoparticles of α-elastin-g-PLGA for potential treatment of restenosis.
Mauro Di StefanoSalvatrice RigogliusoGiovanna PitarresiGiovanna PitarresiFiorica CalogeroFabio Salvatore PalumboGiulio GhersiGaetano GiammonaGaetano Giammonasubject
Myocytes Smooth MusclePharmaceutical ScienceDexamethasoneMuscle Smooth VascularCoronary Restenosischemistry.chemical_compoundPolylactic Acid-Polyglycolic Acid CopolymerDrug DiscoveryMyocyteAnimalsHumansLactic AcidParticle SizeCells CulturedCell ProliferationDrug CarriersbiologyCell growthElastaseBiological activityCell DifferentiationElastinBlotPLGAchemistryBiochemistryBiophysicsbiology.proteinMolecular MedicineNanoparticlesCattleDrug carrierElastinPolyglycolic Aciddescription
A graft copolymer of α-elastin with poly(lactic-co-glycolic) acid (PLGA) has been synthesized and successfully employed to produce nanoparticles. Exploiting the known biological activity of α-elastin to promote the maintenance of smooth muscle cells (SMCs) contractile phenotype and the antiproliferative effect of glucocorticoids, the aim of this research was to produce drug-loaded nanoparticles suitable for potential treatment of restenosis. In particular, nanoparticles of α-elastin-g-PLGA with a mean size of 200 nm have been produced and loaded with dexamethasone dipropionate (10% w/w), chosen as a model drug that inhibits proliferation of vascular SMCs. These nanoparticles are able to prolong the drug release and show a pronounced sensibility to elastase. Drug unloaded nanoparticles stimulate the differentiation of human umbilical artery smooth muscle cells (HUASMCs) toward the contractile phenotype as demonstrated by immunofluorescence, flow cytofluorimetric, and western blotting analyses. Finally, drug-loaded nanoparticles efficiently reduce viability of HUASMCs as evidenced by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay.
year | journal | country | edition | language |
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2013-11-12 | Molecular pharmaceutics |