6533b7defe1ef96bd12768c8
RESEARCH PRODUCT
Cathepsin-B Induced Controlled Release from Peptide-Capped Mesoporous Silica Nanoparticles
Félix SancenónLaura MondragónPedro AmorósCristina De La TorreRamón Martínez-máñezCarmen CollMar OrzáezEnrique Pérez-payáMaría D. Marcossubject
INGENIERIA DE LA CONSTRUCCIONCell Survivalgated mesoporous materialsPeptideAntineoplastic AgentsCatalysisCathepsin BCell LineCathepsin BHeLaQUIMICA ORGANICAHumansCytotoxicityPeptide sequencechemistry.chemical_classificationDrug CarriersbiologyOrganic ChemistryQUIMICA INORGANICAGeneral ChemistryMesoporous silicabiology.organism_classificationSilicon DioxideControlled releasechemistryBiochemistryDoxorubicinBiophysicspeptidesnanoparticlescontrolled releasePorosityIntracellularHeLa Cellsdescription
New capped silica mesoporous nanoparticles for intracellular controlled cargo release within cathepsin B expressing cells are described. Nanometric mesoporous MCM-41 supports loaded with safranin O (S1-P) or doxorubicin (S2-P) containing a molecular gate based on a cathepsin B target peptidic sequence were synthesized. Solids were designed to show "zero delivery" and to display cargo release in the presence of cathepsin B enzyme, which selectively hydrolyzed in vitro the capping peptide sequence. Controlled delivery in HeLa, MEFs WT, and MEFs lacking cathepsin B cell lines were also tested. Release of safranin O and doxorubicin in these cells took place when cathepsin B was active or present. Cells treated with S2-P showed a fall in cell viability due to nanoparticles internalization, cathepsin B hydrolysis of the capping peptide, and cytotoxic agent delivery, proving the possible use of these nanodevices as new therapeutic tools for cancer treatment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |