6533b81ffe1ef96bd1276f53
RESEARCH PRODUCT
false
subject
0303 health sciencesChromatographyPcr cloningPcr assay02 engineering and technologyDna recoveryLiquid nitrogen021001 nanoscience & nanotechnologyGeneral Biochemistry Genetics and Molecular BiologyDna detection03 medical and health scienceschemistry.chemical_compoundchemistryDigital polymerase chain reactionDroplet microfluidics0210 nano-technologyDNA030304 developmental biologyBiotechnologydescription
Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-12-01 | BioTechniques |