6533b81ffe1ef96bd127706d

RESEARCH PRODUCT

Analyzing Oligomerization of Individual Transmembrane Helices and of Entire Membrane Proteins in E. coli: A Hitchhiker’s Guide to GALLEX

Charles R. SandersFlorian CymerDirk Schneider

subject

Transmembrane domainMembraneMembrane proteinStructural biologyCytoplasmmedicineBiophysicsBiological membraneBiologyBioinformaticsmedicine.disease_causeEscherichia coliTransmembrane protein

description

Genetic systems, which allow monitoring interactions of individual transmembrane α-helices within the cytoplasmic membrane of the bacterium Escherichia coli, are now widely used to probe the structural biology and energetics of helix-helix interactions and the consequences of mutations. In contrast to other systems, the GALLEX system allows studying homo- as well as heterooligomerization of individual transmembrane α-helices, and even enables estimation of the energetics of helix-helix interactions within a biological membrane. Given that many polytopic membrane proteins form oligomers within membranes, the GALLEX system represents a unique and powerful approach to monitor formation and stability of oligomeric complexes of polytopic membrane proteins in vivo.

yearjournalcountryeditionlanguage
2012-01-01
https://doi.org/10.1007/978-1-62703-065-6_16