Hitozāna pārklājuma ietekme uz ādas mezenhimālo cilmes šūnu fenotipu 3D šūnu kultūrā

6533b81ffe1ef96bd12775bf

RESEARCH PRODUCT

Hitozāna pārklājuma ietekme uz ādas mezenhimālo cilmes šūnu fenotipu 3D šūnu kultūrā

Rebeka Rožkalne

subject

marķieru ekspresija hitozāns Farmācija mezenhimālās cilmes šūnas 3D kultūra

description

3D šūnu kultūras daudz precīzāk atspoguļo šūnu mijiedarbību in vivo, salīdzinot ar standarta 2D kultūrām. Hitozāns ir unikāls biomateriāls, kurš tiek izmantots 3D šūnu kultūru inducēšanai. Bakalaura darba mērķis bija analizēt hitozāna ietekmi uz 3D mezenhimālo cilmes šūnu (MCŠ) fenotipu. 3D kultūra tika inducēta, izmantojot hitozāna pārklājumu. MCŠ dzīvotspēja, virsmas marķieru CD73, CD90, CD105, nervu kores marķiera CD271, hematopoēzes marķieru CD34, CD45 un pluripotences marķieru SSEA-1, SSEA-4, Oct ¾ ekspresija tika analizēta ar plūsmas citometrijas metodi. MCŠ dzīvotspēja 2D un 3D kultūrā būtiski neatšķīrās. Pēc 72h kultivēšanas 3D, CD90 un CD73 ekspresija MCŠ nokritās par 43% un 54%. Hitozāns inducēja CD271 ekspresiju 2 % D6 MCŠ pēc 24h kultivēšanas 3D. SSEA-4 ekspresijas izmaiņas 3D kultūrā bija atkarīgas no izmantotās MCŠ kultūras.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/36804

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
  - PE2_2 Phenomenology of fundamental interactions
  - PE2_3Experimental particle physics with accelerators
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  - PE2_6Nuclear, hadron and heavy ion physics
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  - PE2_10Atomic, molecular physics
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  - PE2_18Equilibrium and non-equilibrium statistical mechanics: steady states and dynamics
  - PE2_14Lasers, ultra-short lasers and laser physics
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- PE1Mathematics
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LSLife Sciences
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SHSocial Sciences and Humanities
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