6533b81ffe1ef96bd1277a30
RESEARCH PRODUCT
Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana
Sabine WulfertMaría Flores-torneroArmand D. AnomanRuben Maximilian BensteinStephan KruegerRoc Rossubject
0106 biological sciences0301 basic medicinechemistry.chemical_classificationbiologyChemistryMetabolismbiology.organism_classificationPhotosynthesis01 natural sciencesAmino acidSerine03 medical and health scienceschemistry.chemical_compound030104 developmental biologyBiochemistryBiosynthesisBotanyPhotorespirationArabidopsis thalianaPhosphorylation010606 plant biology & botanydescription
Photorespiration is an essential pathway in photosynthetic organisms and is particularly important to detoxify and recycle 2-phosphoglycolate (2-PG), a by-product of oxygenic photosynthesis. The enzymes that catalyze the reactions in the photorespiratory core cycle and closely associated pathways have been identified; however, open questions remain concerning the metabolic network in which photorespiration is embedded. The amino acid serine represents one of the major intermediates in the photorespiratory pathway and photorespiration is thought to be the major source of serine in plants. The restriction of photorespiration to autotrophic cells raises questions concerning the source of serine in heterotrophic tissues. Recently, the phosphorylated pathway of serine biosynthesis has been found to be extremely important for plant development and metabolism. In this protocol, we describe a detailed methodological workflow to analyze the generative and vegetative phenotypes of plants deficient in the phosphorylated pathway of serine biosynthesis, which together allow a better understanding of its function in plants.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-01 |