6533b81ffe1ef96bd12786b4
RESEARCH PRODUCT
Evanescent wave-initiated photopolymerisation as a new way to create monolithic open-tubular capillary columns: use as enzymatic microreactor for on-line protein digestion
Oksana YavorskaFrantišek ForetPetr SmejkalSilvija AbeleSilvija AbeleMirek Mackasubject
Evanescent waveUltraviolet RaysProtein digestionCapillary actionBiochemistryMass SpectrometryAnalytical ChemistryBioreactorsLight sourceCapillary ElectrochromatographyElectrochemistryAnimalsEnvironmental ChemistryHorsesPorous layerPolytetrafluoroethyleneSpectroscopyChromatographyMyoglobinChemistryProteinsSilicon DioxidePepsin ALine (electrical engineering)EnzymesChemical engineeringSeparation methodMicroreactorPorositydescription
Evanescent wave-initiated photopolymerisation in an optically wave guiding PTFE-coated fused silica capillary using light-emitting diode as a light source, is established here as a way to fabricate monolithic porous layer open-tubular capillary columns with a potential in capillary separation methods; application of the obtained open-tubular columns as enzymatic microreactors for on-line protein digestion is demonstrated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-02-23 | The Analyst |