Genomiskā nestabilitāte in vitro krūts vēža šūnu līnijās MDA-MD-436, MDA-MB-231 un SkBr3 hroniskas hipoksijas gadījumā

6533b81ffe1ef96bd1278ac5

RESEARCH PRODUCT

Genomiskā nestabilitāte in vitro krūts vēža šūnu līnijās MDA-MD-436, MDA-MB-231 un SkBr3 hroniskas hipoksijas gadījumā

Marta Priedīte

subject

hipoksija krūts vēzis normoksija genomiskā nestabilitāte Bioloģija

description

Hipoksijas apstākļi audzēja mikrovidē var stimulēt genoma nestabilitāti, tādējādi veicinot agresīvāku slimības gaitu. Darba mērķis bija noteikt vieglas hroniskas hipoksijas ietekmi uz genoma nestabilitāti trīs dažādās krūts vēža šūnu līnijās. Šūnas tika kultivētas trīs pārsējumos hipoksijā (2 % O_2) un normoksijā (19, 95 % O_2). Vieglas hroniskas hipoksijas apstākļos tika novērota augstāka ar DNS reparācijas mehānismiem saistīto signālceļu gēnu ekspresija, palēnināta šūnu proliferācija, izmaiņas morfoloģijā, kā arī izmaiņas šūnas ciklā. Lielākās izmaiņas novērotas mazāk diferencētā šūnu līnijā (MDA-MB-436). Darbs izstrādāts RSU Onkoloģijas institūta Molekulārās ģenētikas laboratorijā no 2016. gada jūlija līdz 2017. gada maijam.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/36182

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