6533b81ffe1ef96bd1278f9a
RESEARCH PRODUCT
Identification of four distinct subunit types in the unique 6 x 6 hemocyanin of the centipede Scutigera coleoptrata.
W. GebauerJ. Marklsubject
biologyCrossed immunoelectrophoresisStereochemistryMacromolecular Substancesmedicine.medical_treatmentProtein subunitNative Polyacrylamide Gel ElectrophoresisHemocyaninGeneral Medicinebiology.organism_classificationMolecular biologychemistry.chemical_compoundElectrophoresischemistryHemolymphHemocyaninsmedicineAnimalsElectrophoresis Polyacrylamide GelSodium dodecyl sulfateCentipedeArthropodsEcology Evolution Behavior and SystematicsScutigera coleoptratadescription
We isolated 6 x 6 hemocyanin, dissociated it into subunits, and examined it by electron microscopy. The subunits were separated by native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate PAGE, and crossed immunoelectrophoresis. Single subunits were isolated by gel cutting from native PAGE and identified as hemocyanin by measuring their ultraviolet spectrum. A total of four distinct hemocyanin subunits were identified, and the subunit pattern of the three electrophoresis systems assigned to each other. The relative proportion of subunits a:b:c:d were 2 : 2 :: 1 as determined by densitometry. Presumably, c and d act as linkers between hexamers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-09-10 | Die Naturwissenschaften |