6533b820fe1ef96bd12791b7
RESEARCH PRODUCT
Nucleotide sequence of plasmid p4028, a cryptic plasmid from Leuconostoc oenos.
Sergi FerrerIsabel PardoManuel Zúñigasubject
GeneticspBluescriptbiologyBase SequenceATPaseGenetic VectorsMolecular Sequence DataRestriction MappingNucleic acid sequenceMolecular biologyOpen reading frameOpen Reading FramesPlasmidCryptic plasmidBacterial ProteinsGenBankbiology.proteinAmino Acid SequenceCloning MolecularMolecular BiologyLeuconostocPlasmidsdescription
Abstract TheLeuconostoc oenosplasmid p4028 was cloned in pBlueScript (SK+), and its complete nucleotide sequence was determined. The analysis of the nucleotide sequence revealed five open reading frames, all of them located on the same strand and grouped in two clusters separated by a short noncoding stretch. A similarity search against the other sequences deposited in the EMBL and GenBank databases showed that p4028 has no significant similarity with any of the sequences checked. Nevertheless, a putative ATP-binding motif was found in ORF2. A more detailed analysis of this ORF suggests that it could encode for a DNA-dependent ATPase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-07-01 | Plasmid |