6533b820fe1ef96bd12791e9

RESEARCH PRODUCT

Sequence analysis of the rDNA spacer of Paracentrotus lividus and observations about pre-rRNA processing. NTS sequence of Paracentrotus lividus rDNA.

Maria CantoneGiovanni GiudiceRainer BarbieriV. IzzoG. Duro

subject

Sequence analysisMolecular Sequence DataRestriction MappingDNA RibosomalParacentrotus lividusIntergenic regionSpecies SpecificitySequence Homology Nucleic AcidGeneticsRNA PrecursorsAnimalsRNA Processing Post-TranscriptionalRRNA processingMolecular BiologyRibosomal DNAbiologyBase SequenceGeneral MedicineSpacer DNARibosomal RNAbiology.organism_classificationMolecular biologyExternal transcribed spacerSea UrchinsOocytesFemale

description

We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor while has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.

yearjournalcountryeditionlanguage
1993-10-01Molecular biology reports
10.1007/bf01674428https://pubmed.ncbi.nlm.nih.gov/8114685