6533b820fe1ef96bd12798c7
RESEARCH PRODUCT
Indicator-free electrochemical DNA hybridization biosensor
João Roberto FernandesMian JiangGustavo A. RivasJoseph WangRussel WaymireJose Luis Lopez PazJose Luis Lopez Pazsubject
Detection limitChromatographyChemistryGuanineDNA–DNA hybridizationAnalytical chemistryElectrochemistryBiochemistryAnalytical ChemistryDna hybridization biosensorchemistry.chemical_compoundTransducerEnvironmental ChemistryMolecular probeBiosensorSpectroscopydescription
A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a flat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-11-01 | Analytica Chimica Acta |