6533b820fe1ef96bd1279ba6

RESEARCH PRODUCT

A Quantitative In Vitro Approach to Study the Intracellular Fate of Gold Nanoparticles: From Synthesis to Cytotoxicity

Uwe HolzwarthSabrina GioriaFrançois RossiChiara UboldiJessica PontiC. James KirkpatrickRenato ColognatoFederica SimonelliKamel AbbasFabio Franchini

subject

Neutral redMaterials sciencemedia_common.quotation_subject[SDV]Life Sciences [q-bio]CellBiomedical EngineeringNanotechnologymedicine.disease_causeToxicologychemistry.chemical_compoundmedicine[CHIM]Chemical SciencesCytotoxicityInternalizationComputingMilieux_MISCELLANEOUSmedia_commonIntracellular fate[CHIM.MATE]Chemical Sciences/Material chemistryIn vitromedicine.anatomical_structurechemistryColloidal gold[SDV.TOX]Life Sciences [q-bio]/ToxicologyBiophysics[CHIM.RADIO]Chemical Sciences/RadiochemistryGenotoxicity

description

Due to their physico-chemical characteristics, gold nanoparticles (AuNPs) seem to be suitable for biomedical and therapeutic applications even if conflicting data on their toxicological profiles are present in literature. In order to better understand if AuNPs could be safe we must consider different biological endpoints such as cytotoxicity, genotoxicity, inflammation and biopersistence. Starting from these considerations, one of the first issues to be assessed is to better understand if AuNPs can be internalized by cells. In this work, we propose a methodological approach to radioactivate AuNPs by neutron activation and the quantification of their internalization by two in vitro cell systems such as MDCK and HepG2 after 24 h of exposure. Despite a dose-dependent internalization, no evidence of cytotoxicity, determined by two different standard in vitro methods such as Neutral Red Uptake and Colony Forming Efficiency, was observed.

10.1080/17435390903056384http://publications.jrc.ec.europa.eu/repository/handle/JRC54462