6533b820fe1ef96bd127a392

RESEARCH PRODUCT

Analysis of the polypeptide composition of the cell walls of Neurospora crassa. Similarities with the proteinaceous material secreted by the slime variant

J. Ruiz-herreraM L GilJosé P. MartínezRafael SentandreuManuel Casanova

subject

Strain (chemistry)biologyPlant Sciencebiology.organism_classificationNeurospora crassaStainingCell wallBiochemistryConcanavalin AGeneticsbiology.proteinExtracellularSecretionPolyacrylamide gel electrophoresisEcology Evolution Behavior and SystematicsBiotechnology

description

The polypeptide composition of cell walls from the wild-type strain of Neurospora crassa is compared with that of the proteinaceous extracellular coat (PEM) secreted by the slime strain of this fungus. Analyses included determination of the polypeptide pattern by polyacrylamide gel electrophoresis and blotting followed by staining with Concanavalin A and antibodies raised against the overall antigenic components present in either (whole cell walls or PEM) structure. A complex protein assortment was found associated to the walls of the wild type strain. The similarities observed between the polypeptide patterns of the cell walls and PEM, in addition to the immunological cross-reactivity exhibited by some proteinaceous species, suggest a close relationship between PEM and the proteins present in the wild-type cell walls.

yearjournalcountryeditionlanguage
1991-03-01Mycological Research
https://doi.org/10.1016/s0953-7562(09)81240-9