6533b820fe1ef96bd127a449
RESEARCH PRODUCT
Natural co-occurrence of ochratoxin A, ochratoxin B and aflatoxins in Sicilian red wines
Rosa PitonzoGiuseppe AvelloneGiacomo DugoNicola CiceroValentina Giusi CapocchianoVita Di StefanoAlessia Mazzasubject
Ochratoxin AAflatoxinAflatoxin B1Health Toxicology and MutagenesisOchratoxin BFood ContaminationWineAverage levelaflatoxin G2Toxicologyaflatoxin G1Sensitivity and SpecificityFluorescencechemistry.chemical_compoundAflatoxinsimmunoaffinity columnFood scienceHPLC/FLD analysiMycotoxinSicilyChromatography High Pressure LiquidWineChromatographyPublic Health Environmental and Occupational HealthGeneral ChemistryGeneral MedicineOchratoxinsimmunoaffinity column wine aflatoxin B-2 aflatoxin G(1) ochratoxin B ochratoxin A aflatoxin B-1 aflatoxin G(2) HPLC/FLD analysischemistryaflatoxin B2Maximum Allowable Concentrationochratoxin Aochratoxin BFood Sciencedescription
The natural occurrence of ochratoxin A, ochratoxin B, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 (OTA, OTB, AFB1, AFB2, AFG1, AFG2) in red wines was investigated by HPLC/FLD after immunoaffinity column clean-up in 57 market samples produced in Sicily (Italy). The results showed a very low incidence of these mycotoxins in analysed samples, confirming the high degree of quality and safety of Sicilian red wines. The results indicated 71.9% and 64.9% positive samples for OTA and OTB respectively, with an average level of 0.13 μg l(-1), well below the European maximum permitted levels (MLs). The aflatoxin most frequently detected in the samples was AFG1, present in 57.9% of samples, while the other aflatoxins were rarely present. Recovery experiments were carried out on eight mycotoxin-free red wines spiked with OTA, OTB, AFB1, AFB2, AFG1 and AFG2 at two different levels. The limits of detection (LODs) in wines were 0.02 µg l(-1) for OTA, 0.04 µg l(-1) for OTB, 0.03 µg l(-1) for AFG1, AFG2 and AFB2, and 0.05 µg l(-1) for AFB1. A good correlation was found, with good performances in term of precision for the method.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-01 |