6533b820fe1ef96bd127a49a

RESEARCH PRODUCT

Uptake of [3H]dopamine into dopaminergic and noradrenergic neurones of the isolated neurointermediate lobe of the rat hypophysis. Effects of desipramine and nomifensine.

Kurt RackéErich Muscholl

subject

Malemedicine.medical_specialtyNomifensineDopamineBiochemistryCellular and Molecular NeuroscienceNorepinephrineNorepinephrinePituitary Gland PosteriorDopamineInternal medicineDesipraminemedicineAnimalsCatecholamine uptakeNeuronsChemistryDopaminergicDesipramineNeurointermediate lobeIsoquinolinesRatsNomifensineEndocrinologyCatecholaminemedicine.drug

description

The isolated neurointermediate lobe (NIL) of the rat hypophysis accumulates [3H]dopamine from the incubation medium. Column chromatographic analysis showed that 92% of the tissue radioactivity was contained in the catecholamine fraction. [3H]Dopamine represented 70% and [3H]noradrenaline 30% of the [3H]catecholamines. Desipramine (1 microM) prevented the formation of [3H]noradrenaline without affecting the storage of [3H]dopamine. Nomifensine (10 microM) blocked the storage of [3H]dopamine and [3H]noradrenaline. Thus, in the NIL, [3H]dopamine is taken up into dopaminergic and noradrenergic neurones. In the latter, [3H]dopamine is converted to [3H]noradrenaline, indicating a significant dopamine beta-hydroxylase activity in the NIL tissue. A selective labeling of the dopamine stores with [3H]dopamine can be achieved in the presence of desipramine.

yearjournalcountryeditionlanguage
1983-11-01Journal of neurochemistry
10.1111/j.1471-4159.1983.tb00850.xhttps://pubmed.ncbi.nlm.nih.gov/6619880