6533b820fe1ef96bd127a6ad

RESEARCH PRODUCT

Use of two-dimensional thin-layer chromatography for the components study of poly(adenosine diphosphate ribose)

J. DesgrèsGérard KeithG. De Murcia

subject

chemistry.chemical_classificationPoly Adenosine Diphosphate RiboseChromatographyPolymersChemistryBiophysicsPoly Adenosine Diphosphate RiboseCell BiologyBranching pointsPolymerBiochemistryAdenosineAdenosine MonophosphateIn vitroThin-layer chromatographyVenom phosphodiesterasechemistry.chemical_compoundmedicineElectrophoresis Polyacrylamide GelChromatography Thin LayerCelluloseMolecular Biologymedicine.drug

description

Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly(ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly(ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length. (ii) The branching points are located at regular distances of approximately 41 derivatives from each other.

yearjournalcountryeditionlanguage
1990-12-01Analytical Biochemistry
https://doi.org/10.1016/0003-2697(90)90224-w