Hepatīta B vīrusa G genotipa serdes antigēna veidoto vīrusveidīgo daļiņu raksturošana

6533b820fe1ef96bd127a80f

RESEARCH PRODUCT

Hepatīta B vīrusa G genotipa serdes antigēna veidoto vīrusveidīgo daļiņu raksturošana

Jānis Pjalkovskis

subject

disociācija ar guanidīna hidrohlorīdu HBcAg Bioloģija HBV G genotips sārmu hidrolīzes metode vīrusveidīgās daļiņas

description

Šajā darbā tika salīdzinātas divas metodes HBV serdes antigēna veidoto vīrusveidīgo daļiņu (VVD) attīrīšanai no iepakotajām nukleīnskābēm – metode kapsīdu disociācijai ar GuHCl un sārmu hidrolīzes metode. Darbā aprakstīti un analizēti abu attīrīšanas metožu posmi un izdarīti secinājumi par attiecīgo attīrīšanas metožu efektivitāti. Tāpat tika raksturotas ar abām attīrīšanas metodēm iegūtās HBV G genotipa serdes antigēna VVD, nosakot to morfoloģiju (elektronu mikroskopija, dinamiskā gaismas izkliede) un spēju pakot raksturotas funkcionālas nukleīnskābes – CpG oligonukleotīdus, bakteriofāga dsRNS un DTxA mRNS. Eksperimentu rezultāti parāda, ka ar abām attīrīšanas metodēm iegūtās tukšās VVD spēj pakot nukleīnskābes. Ar sārmu hidrolīzi attīrīto VVD diametrs ir lielāks, kā ar GuHCl attīrīto VVD. Attīrīšanas metožu analīze parāda augstāku proteīna iznākumu attīrot disociācijā ar GuHCl, bet sārmu hidrolīzes metode ir viegli pārveidojama un uzlabojama.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/32194

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