6533b821fe1ef96bd127abef
RESEARCH PRODUCT
Isolierung und charakterisierung einer cholinkinase aus Phaseolus vulgaris L.-Keimlingen
W. SchleicherElmar Hartmannsubject
chemistry.chemical_classificationCholine kinasebiologyChemistryGeneral Medicinebiology.organism_classificationEnzyme assayHypocotylPhosphotransferasechemistry.chemical_compoundEnzymeBiochemistrybiology.proteinCholinePhaseolusGibberellic aciddescription
Summary The enzyme choline kinase (ATP: Choline phosphotransferase E.C. 2.7.1.32) was extracted and partially purified from hypocotyl hooks of Phaseolus vulgaris L. seedlings. K m -value and pH-dependence of the activity were determined. The amount of enzyme activity in extracts depended on light conditions used for plant growth. Etiolated seedlings showed much lower enzyme levels than those grown in white light. Blue and red light conditions decreased enzyme levels below dark values. The in vitro enzyme activity was influenced by inhibitors and growth regulators. The enzyme activity was stimulated by Atropine, 2-Chloroethylammoniumchloride (Cycocel) and Gibberellic acid and was inhibited by Physostigmine. Neostigmine had no effects.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1977-06-01 | Zeitschrift für Pflanzenphysiologie |