6533b821fe1ef96bd127afd2
RESEARCH PRODUCT
Reversion of 7-methylguanosine 5′-phosphate inhibition of mRNA translation by polysomal and soluble factors isolated from Saccharomyces cerevisiae
Antonio Parets SolerJesús ZuecoDaniel GozalboRafael Sentandreusubject
RNA CapsSucroseSaccharomyces cerevisiaeBiophysicsReversionSaccharomyces cerevisiaeRNA Cap AnalogsBiochemistryFungal Proteinschemistry.chemical_compoundReticulocytePolysomemedicineRNA MessengerMolecular BiologyMessenger RNAbiologyTranslation (biology)Cell Biologybiology.organism_classificationMolecular biologyYeastKineticsmedicine.anatomical_structurechemistryBiochemistryPolyribosomesProtein Biosynthesisdescription
Abstract Protein fractions that overcome m7GMP inhibition of mRNA translation have been purified from the yeast S. cerevisiae . An active fraction isolated from polysomes contains two polypeptides of 220- and 190-kDa. The active fraction isolated from postribosomal supernatant contains a major polypeptide of 28-kDa and other species of 32-, 24-, 22- and 21-kDa, and sediments in sucrose gradients as a high molecular weight complex of about 200000. This fraction restored yeast mRNA translation in reticulocyte lysates under conditions of yeast and globin mRNA competition; however, this effect was not observed with the 220- and 190-kDa polypeptides from polysomes. Nevertheless, translation of yeast mRNA was stimulated by a partially purified fraction containing a 28-kDa polypeptide from polysomes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-08-01 | Biochemical and Biophysical Research Communications |