6533b821fe1ef96bd127b64f
RESEARCH PRODUCT
The comparative behavior of Lactococcus lactis in free and immobilized culture processes
R CachonP AntérieuxC Divièssubject
chemistry.chemical_classificationbiologyLactococcusIntracellular pHLactococcus lactisBioengineeringGeneral Medicinebiology.organism_classificationApplied Microbiology and Biotechnologychemistry.chemical_compoundEnzymechemistryBiochemistryLactate dehydrogenaseBioreactorNAD+ kinaseIntracellularBiotechnologydescription
This study presents the changes in the physiological state of Lactococcus lactis in different actively growing systems. The intracellular pH (pHin), NADH/NAD ratio and lactate dehydrogenase (LDH) activity were measured in free cell batch culture with or without external pH (pHout) control, and in continuous free or immobilized cell reactors. In free cell batch culture it has been shown that the pHin is dependent both on pHout and age of the culture, while in free cell continuous culture the pHin and the pHout are close together. The NADH/NAD ratio varied inversely with the growth rate, which was related to the changes in the metabolic activities in lactococci. The specificity of the immobilized cell technology has been characterized, the properties of realized cells followed those of immobilized cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-08-01 | Journal of Biotechnology |