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RESEARCH PRODUCT

Application of Nanogen microarray technology for forensic SNP analysis

M.k. BaloghKlaus BenderPeter M. Schneider

subject

GeneticsFully automatedPcr cloningGene chip analysisGeneral MedicineComputational biologyBiologyDNA microarraySNP arrayHigh throughput analysis

description

Abstract The NanoChip® Molecular Biology Workstation using electronic microarrays is an approach for rapid and high throughput analysis of SNPs. This instrument is fully automated and uses a microchip for electronic addressing of capture probes to specific array sites followed by electronic hybridisation of the single stranded PCR products, and passive hybridisation of fluorescently labelled reporter probes. Discrimination is achieved by applying thermal stringency to denature the mismatched reporters. 48 SNP assays have been designed using the ‘capture down’ assay which applies a thermal ‘touch down’ strategy to obtain the best reporter probe discrimination.

yearjournalcountryeditionlanguage
2006-04-01International Congress Series
https://doi.org/10.1016/j.ics.2005.10.047