6533b821fe1ef96bd127b851

RESEARCH PRODUCT

Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae

Lobna Abdelkefi-mesratiSaoussen Ben KhedherNesrine TrikiMaissa ChakrounHanen BoukediImen SaadaouiSlim TounsiFakher Kamoun

subject

InsecticidesEctomyelois ceratoniaebiologyBrush borderToxinBacillus thuringiensisMidgutMothsbiology.organism_classificationmedicine.disease_causeMicrobiologyBacterial ProteinsVacuolizationBacillus thuringiensismedicineAnimalsOverproductionEscherichia coliEcology Evolution Behavior and Systematics

description

Abstract The vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli . Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. Accordingly, the optimum Vip3A16 toxin production was 170 μg/ml at 18 h post-induction time and 39 °C post-induction temperature. This corresponds to an improvement of 21 times compared to the starting conditions. The insecticidal activity, evaluated against Ectomyelois ceratoniae , displayed an LC 50 value of 40 ng/cm 2 and the midgut histopathology of Vip3Aa16 fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical region and cellular disintegration.

yearjournalcountryeditionlanguage
2015-02-02Journal of Invertebrate Pathology
https://doi.org/10.1016/j.jip.2015.03.013