6533b821fe1ef96bd127b8af
RESEARCH PRODUCT
Structure of the phenylalanine hydroxylase gene in Drosophila melanogaster and evidence of alternative promoter usage.
Mireille MoulardFrancisco J. SilvaPilar Ruiz-vázquezsubject
Phenylalanine hydroxylaseTranscription GeneticMolecular Sequence DataBiophysicsGenes InsectBiochemistryPolymerase Chain ReactionExonchemistry.chemical_compoundAromatic amino acidsAnimalsGenomic libraryAmino Acid SequenceRNA MessengerPromoter Regions GeneticMolecular BiologyGeneDNA PrimersGeneticsGenomic LibrarybiologyBase SequenceIntronPhenylalanine HydroxylaseCell BiologyExonsbiology.organism_classificationMolecular biologyIntronsOpen reading frameDrosophila melanogasterchemistrybiology.proteinDrosophila melanogasterdescription
The complete Drosophila melanogaster phenylalanine hydroxylase gene isolated from a genomic library was sequenced. Gene structure consisted of five exons covering a region of around 3 kb. Position of introns in the C-terminal domain was conserved with mammalian aromatic amino acid hydroxylase genes. Putative promoter sequences in the 5'UTR and intron 1 were identified. A novel transcript was detected differing from that previously reported by the inclusion of a part of the intron 1 sequence. It could be produced using an alternative promoter. The deduced open reading frame would code a protein with a small difference at the N-terminus. Expression of the alternative transcripts was examined throughout development.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-08-05 | Biochemical and biophysical research communications |