6533b821fe1ef96bd127b94b

RESEARCH PRODUCT

Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

Lennart KenneTomasz NiedzielaCzeslaw LugowskiCzeslaw Lugowski

subject

Magnetic Resonance SpectroscopyStereochemistryMolecular Sequence DataSize-exclusion chromatographyPolysaccharideBiochemistryAnalytical ChemistryHydrolysischemistry.chemical_compoundMonosaccharidechemistry.chemical_classificationTeichoic acidbiologyHydrolysisOrganic ChemistryO AntigensHafnia alveiGeneral MedicineNuclear magnetic resonance spectroscopyHafniabiology.organism_classificationTeichoic Acidscarbohydrates (lipids)Carbohydrate SequencechemistryBiochemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPhosphodiester bond

description

Abstract The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by 1 H, 13 C, and 31 P NMR spectroscopy, MALDI-TOF MS, and GC–MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {→4-α- d -Glc p -(1→3)-α- l - Fuc p NAc-(1→3)-[α- d -Glc p -(1→4)]-α- d -Glc p NAc-(1→3)-α- l - Fuc p NAc-(1→4)-α- d -Glc p -(1→P} n .

yearjournalcountryeditionlanguage
2009-07-16Carbohydrate Research
https://doi.org/10.1016/j.carres.2009.11.005